Golf Course Management

FEB 2016

Golf Course Management magazine is dedicated to advancing the golf course superintendent profession and helping GCSAA members achieve career success.

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102 GOLF COURSE MANAGEMENT 02.16 conducted on 3-week-old perennial ryegrass seedlings of the same cultivar blend on which the disease was initially observed. This blend was grown in 4-inch-diameter (10-cm) pots containing commercial planting mix. One 0.16-inch-diameter (4-mm) plug was removed from the margin of a 4-day-old colony grow - ing on PDA and placed on wet leaves. Five replicate pots of each inoculated and non- inoculated ryegrass seedlings were kept in a moist chamber at 72 F (22 C). Within four to fve days, inoculated leaves exhibited slight chlorosis, and water-soaked lesions with white aerial mycelium were present. Koch's postu - lates were fulflled as mycelia were recovered from diseased lesions, and were confrmed through amplifcation and sequencing of the same ITS region. This demonstrates that W. circinata is pres - ent in Oklahoma and in the southern Midwest. To our knowledge, this is the frst report of W. circinata infecting perennial ryegrass in this re - gion. Given that it has not been previously re- ported in this region, turfgrass managers may not be correctly identifying this disease. Source: Accepted for publication in Plant Disease Nathalia Graf Grachet ( is a graduate student and Nathan Walker ( is a professor in the Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater. First report of Xanthomonas translucens causing wilt disease on perennial ryegrass in the United States P.R. Giordano, Ph.D.; Q. Zeng, Ph.D.; N.M. Dykema; A.R. Detweiler; and J.M. Vargas Jr., Ph.D. Bacterial diseases of turfgrass are rare, but can be devastating because of their prolifc na - ture and the lack of labeled effcacious pesti- cides. Bacterial wilt of perennial ryegrass (Lo- lium perenne) was frst observed on a golf course fairway in Baltimore, Md., during hot (30 C to 33 C), humid weather in May 2013. Symptoms of general wilt, etiolation, chlorosis and decline were observed on individual plants in irregular (report) Perennial ryegrass afficted with wilt disease caused by Xanthomonas translucens was found in Baltimore. Photo by Eric David

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