Golf Course Management

JAN 2013

Golf Course Management magazine is dedicated to advancing the golf course superintendent profession and helping GCSAA members achieve career success.

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research root tissue lacked a posterior cone-like protuberance of the vulva typical of M. graminis. DNA was extracted from 15 single juveniles, and mitochondrial DNA testing showed that the nematodes were genetically similar to M. marylandi. Comparisons of additional genetic sequencing showed that the 15 juveniles had a 98% sequence similarity with M. marylandi. Although M. marylandi has been reported on bermudagrass in many areas of the United States and other places throughout the world, to our knowledge, this is the ���rst detection of this nematode in Florida. Further studies will be conducted to determine the prevalence, incidence and severity of damage caused by M. marylandi, and to determine a possible mode of dispersal on turfgrasses. Source: Plant Disease, October 2012, 96(10):1583. http://dx.doi.org/10.1094/PDIS-06-12-0544-PDN N.S. Sekora (nssekora@u���.edu) is a postdoctoral associate, W.T. Crow is an associate professor, and T. Mekete is assistant professor in the entomology and nematology department, University of Florida, Gainesville. V V V Report First report of dollar spot caused by Sclerotinia homoeocarpa on creeping bentgrass in North Dakota Jared M. LeBoldus, Ph.D.; Qi Zhang, Ph.D.; and Kasia Kinzer, M.S. Dollar spot disease is a major concern for golf courses nationwide, resulting in poor turf quality and signi���cant damage to playing surfaces. To manage this disease effectively, fungicides need to be applied on a biweekly basis. This management strategy represents a signi���cant cost to turfgrass managers and may negatively affect the economics of the industry in North Dakota. In the summer of 2011, small circular, sunken brown patches of dead turf approximately 2 inches (5 centimeters) in diameter, resembling dollar spot, were observed on a creeping bentgrass (Agrostis stolonifera L.) variety trial at the North Dakota State University Agricultural Experiment Station in Fargo, N.D. Fresh individual leaf specimens with distinct lesions having straw-colored centers and reddish brown margins were collected. Leaves were surface disinfected in a 0.05% sodium chloride solution for 60 seconds, rinsed three times in sterile distilled water, then placed onto potato dextrose agar. Three isolates were obtained from the disease-infested leaves with morphology similar to that described for Sclerotinia homoeocarpa F.T. Bennett. Fungal colonies were initially colorless but developed sparse white Sclerotinia homoeocarpa is the causal agent of dollar spot on turfgrass. Photos by Jared M. LeBoldus Small circular, sunken brown patches of dead turf approximately 2 inches in diameter can be evidence of dollar spot on creeping bentgrass. January 2013 GCM 139

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